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Nature based synergy
Fungal Endophyte
Plant Root
BasidioBoost is the next-generation microbial solution designed to significantly boost plant health and root development through advanced endophytic fungal hyphae. BasidioBoost enhance phosphorus uptake leads to increased nodule formation in soybeans (dicots) and greater biomass and yield production in wheat (monocots).
Powering Plants Through Phosphorus
BasidioBoost fungal strains represent a 4th generation advancement in bio fertility, offering improved P assimilation. BasidioBoost could be applied with high concentration of several phosphorus fertilizer including MAP, DAP, SSP and TSP.
What makes it revolutionary?
Traditional microbes often fall short when exposed to modern agricultural conditions like chemical fertilizers or limited nutrient availability. BioSam’s proprietary endophytic fungi—BasidioBoost.1 and BasidioBoost.2—are engineered by nature and optimized by science to thrive in real-world soil conditions, mobilizing phosphorus where it matters most: in the root zone.
The Science in Action
When applied to crops, our fungi colonize plant roots quickly and efficiently. This active colonization triggers two powerful outcomes:
Soybeans (dicots): show increased nodule formation, a strong biological signal of enhanced nitrogen fixation, resulting in healthier, more productive plants.
Wheat (monocots) : displays significant biomass gain, a direct reflection of improved phosphorus availability and overall growth acceleration. Compared to mock-inoculated controls, the difference is clear, consistent, and quantifiable—with enhanced nutrient uptake translating into robust above-ground growth and stronger yields.
Why does it matter
In a world striving for sustainability, the ability to naturally boost productivity without synthetic overload is a game-changer. BioSam’s solution works with the plant, for the soil, and toward a healthier planet.
BasidioBoost Reduces N₂O Emissions Compared to Conventional Fertilization
This figure shows that BasidioBoost, a microbial biofertilizer by BioSam, leads to lower nitrous oxide (N₂O) emissions than conventional strip-till fertilization. The graph tracks daily N₂O flux over time, with BasidioBoost (blue line) showing significantly reduced peaks compared to the conventional method (green line). The photo depicts the field measurement setup used in collaboration with Area X.O., Ottawa, Canada. These results demonstrate BasidioBoost’s effectiveness in reducing agricultural greenhouse gas emissions.
BasidioBoost enhances Wheat Yield by improved P assimilation
This image summarizes the positive effects of the BasidioBoost treatment on key wheat yield parameters, evaluated under semi-field conditions. The table quantifies the impact of the treatment, comparing results with and without the presence of T. versicolor fungus under both phosphorus-deprived (C0) and phosphorus-rich (CP) conditions. Measured parameters include tillers per plant, ears per plant, straw yield, grain yield, and barn yield (g/plant). The presented values are means calculated from four replicate pots, each containing eight plants. Highlighted cells in the table indicate statistically significant improvements or key results associated with the BasidioBoost treatment.
| Condition | T. versicolor | Tillers plant | Ears / plant | Straw yield g / plant | Grain yield g / plant | Barn yield g / plant |
|---|---|---|---|---|---|---|
| P-deprived (CO) | - | 3.8 ± 0.26 | 2.2 ± 0.12 | 2.4 ± 0.15 | 0.9 ± 0.13 | 0.75 ± 0.09 |
| P-deprived (CO) | + | 4.8 ± 0.47* | 2.6 ± 0.28* | 3.3 ± 0.38* | 1.43 ± 0.18* | 0.93 ± 0.08* |
| P-rich (CP) | - | 5.8 ± 0,71 | 3.2 ± 0.42 | 6.04 ± 0.83 | 4.3 ± 0.37 | 1.44 ± 0.11 |
| P-rich (CP) | + | 6.8 ± 0.5 | 4 ± 0.17* | 6.01 ± 0.6 | 4.7 ± 0.12* | 1.68 ± 0.08* |
BasidioBoost Benefits :
Higher yields
Reduced input costs
Optimize P use efficiency
Reduces GHG emissions
BasidioBoost N-Fix adds 33% more growth to the fungus
Generation 4 is finally here
Fungal Endophyte
Bacteria
BasidioBoost N-Fix is the next-generation microbial solution designed to significantly boost monocot health and root development through the synergistic action of a fungal endophyte and an endophytic nitrogen-fixing bacterium. BasidioBoost N-Fix delivers significant improvements in both phosphorus (P) and nitrogen (N) content.
Key features of BasidioBoost N-Fix
improved root colonization pattern
BasidioBoost N-Fix leverages a consortium of beneficial bacteria to forge a more resilient symbiotic relationship with plants in the tri-partite system
Optimized Fungal-Root Symbiosis
The bacteria in BasidioBoost N-Fix enhance the nutrient access for the endophytic fungus, reinforcing its association with plant roots, while the fungus, in turn, fosters conditions that promote greater delivery of bacterial cells to the root system.
Reduction of P and N Chemical Fertilizers
BasidioBoost N-Fix supplies both phosphorus (P) and nitrogen (N) to monocots, thereby decreasing the need for chemical phosphorus and nitrogen fertilizers.
Root Colonization Pattern
BasidioBoost N-Fix demonstrates a more than 33% improvement in plant root development compared to BasidioBoost.
Synergistic Nitrogen Fixation for Enhanced Monocot Growth
This chart showcases the significant advancement represented by BasidioBoost, a newly developed consortium combining an atmospheric nitrogen-fixing bacterium with an endophytic fungus. The presented experimental data on wheat, a key monocot crop, clearly illustrates the synergistic benefits of this dual inoculation. Plants treated with the complete BasidioBoost N-Fix as consortium (F+B) exhibit substantially increased root and shoot dry weight compared to control plants (M) or those inoculated with the fungus (F) or bacterium (B) alone. This breakthrough addresses the long- standing challenge of limited symbiotic nitrogen fixation in non-leguminous crops, paving the way for reduced reliance on synthetic nitrogen fertilizers and promoting more sustainable agricultural practices for monocot cultivation. The visualized fungal hyphae within the wheat root further underscore the close biological interaction facilitated by BasidioBoost.
BasidioBoost N-Fix Benefits :
Higher yields
Reduced input costs
Reduces chemical fertilizer
Reduces GHG emissions
Reduces chemical fertilizer
Supply chain security
BioSam represents the cutting edge of Fungal endophytes innovation in agriculture. Our endophytic basidiomycetes significantly improve phosphorus absorption and could pair with nitrogen-fixing bacteria, deliver reliable performance even with fertilizers—something previous generations couldn't achieve. With a broad host range, higher root colonization and faster production cycles, we’re redefining sustainable farming.
Our proprietary fungal strains colonize roots fast and thrive in high-phosphorus environments. They transport nutrients more efficiently, can be mass-produced in record time, and work seamlessly with free-living nitrogen-fixing bacteria. This synergy means healthier plants, higher yields, and a greener planet.
BioSam’s 4th generation technology outperforms bacteria, fungi, and mycorrhizae from previous generations. We’ve unlocked the next level of biofertility: one that’s effective with chemical fertilizers, offers wide host compatibility, and ensures 20–25% phosphorus absorption in untreated conditions.
