Innovation

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WE ARE THE NEXT GENERATION

The Future of Bio fertility: Generation 4 is Here

Engineered by Nature, Perfected by Science

More Than Just a Microbe—A New Standard

discovery

BioSam is an agri-biotech company developing breakthrough microbial endophytes solution to boost crop yields and farm profitability

discovery

BSI001

plant diagonal : 100µm

Fungal Endophyte

Plant Root

BSI001 is the next-generation microbial solution designed to significantly boost plant health and root development through advanced endophytic fungal hyphae. BSI001 enhance phosphorus uptake leads to increased nodule formation in soybeans (dicots) and greater biomass and yield production in wheat (monocots).

BSI001 fungal strains represent a 4th generation advancement in bio fertility, offering improved P assimilation. BSI001 could be applied with high concentration of several phosphorus fertilizer including MAP, DAP, SSP and TSP.

What makes it revolutionary?

Traditional microbes often fall short when exposed to modern agricultural conditions like chemical fertilizers or limited nutrient availability. BioSam’s proprietary endophytic fungi—BSI001.1 and BSI001.2—are engineered by nature and optimized by science to thrive in real-world soil conditions, mobilizing phosphorus where it matters most: in the root zone.

The Science in Action

When applied to crops, our fungi colonize plant roots quickly and efficiently. This active colonization triggers two powerful outcomes: Soybeans (dicots): show increased nodule formation, a strong biological signal of enhanced nitrogen fixation, resulting in healthier, more productive plants. Wheat (monocots) : displays significant biomass gain, a direct reflection of improved phosphorus availability and overall growth acceleration. Compared to mock-inoculated controls, the difference is clear, consistent, and quantifiable—with enhanced nutrient uptake translating into robust above-ground growth and stronger yields.

Why does it matter

In a world striving for sustainability, the ability to naturally boost productivity without synthetic overload is a game-changer. BioSam’s solution works with the plant, for the soil, and toward a healthier planet.

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This image summarizes the positive effects of the BSI001 treatment on key wheat yield parameters, evaluated under semi-field conditions. The table quantifies the impact of the treatment, comparing results with and without the presence of T. versicolor fungus under both phosphorus-deprived (C0) and phosphorus-rich (CP) conditions. Measured parameters include tillers per plant, ears per plant, straw yield, grain yield, and barn yield (g/plant). The presented values are means calculated from four replicate pots, each containing eight plants. Highlighted cells in the table indicate statistically significant improvements or key results associated with the BSI001 treatment.

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Values given are means of four pots (with eight plants per pot)
ConditionT. versicolorTillers plantEars / plantStraw yield g / plantGrain yield g / plantBarn yield g / plant
P-deprived (CO)-3.8 ± 0.262.2 ± 0.122.4 ± 0.150.9 ± 0.130.75 ± 0.09
P-deprived (CO)+4.8 ± 0.47*2.6 ± 0.28*3.3 ± 0.38*1.43 ± 0.18*0.93 ± 0.08*
P-rich (CP)-5.8 ± 0,713.2 ± 0.426.04 ± 0.834.3 ± 0.371.44 ± 0.11
P-rich (CP)+6.8 ± 0.54 ± 0.17*6.01 ± 0.64.7 ± 0.12*1.68 ± 0.08*

Higher yields

Reduced input costs

Optimize P use efficiency

Reduces GHG emissions

discovery

BSI002

BSI002 adds 33% more growth to the fungus

Fungal Endophyte

Bacteria

BSI002 is the next-generation microbial solution designed to significantly boost monocot health and root development through the synergistic action of a fungal endophyte and an endophytic nitrogen-fixing bacterium. BSI002 delivers significant improvements in both phosphorus (P) and nitrogen (N) content.

improved root colonization pattern

BSI002 leverages a consortium of beneficial bacteria to forge a more resilient symbiotic relationship with plants in the tri-partite system

Optimized Fungal-Root Symbiosis

The bacteria in BSI002 enhance the nutrient access for the endophytic fungus, reinforcing its association with plant roots, while the fungus, in turn, fosters conditions that promote greater delivery of bacterial cells to the root system.

Reduction of P and N Chemical Fertilizers

BSI002 supplies both phosphorus (P) and nitrogen (N) to monocots, thereby decreasing the need for chemical phosphorus and nitrogen fertilizers.

Root Colonization Pattern

BSI002 demonstrates a more than 33% improvement in plant root development compared to BSI001.

BSI002
BSI001

This chart showcases the significant advancement represented by BSI001, a newly developed consortium combining an atmospheric nitrogen-fixing bacterium with an endophytic fungus. The presented experimental data on wheat, a key monocot crop, clearly illustrates the synergistic benefits of this dual inoculation. Plants treated with the complete BSI002 as consortium (F+B) exhibit substantially increased root and shoot dry weight compared to control plants (M) or those inoculated with the fungus (F) or bacterium (B) alone. This breakthrough addresses the long- standing challenge of limited symbiotic nitrogen fixation in non-leguminous crops, paving the way for reduced reliance on synthetic nitrogen fertilizers and promoting more sustainable agricultural practices for monocot cultivation. The visualized fungal hyphae within the wheat root further underscore the close biological interaction facilitated by BSI001.

root dry weight

shoot dry weight

M: Mock
F: Fungus
B: Bacterium

Higher yields

Reduced input costs

Reduces chemical fertilizer

Reduces GHG emissions

Reduces chemical fertilizer

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